BCH210H1 Lecture Notes - Lecture 30: Affinity Chromatography, Electrochemical Gradient, Intermembrane Space

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taupegoldfish44

31 Jan 2018

School

UTSG

Department

Biochemistry

Course

BCH210H1

Professor

Stavroula Andreopoulos

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BCH210H1 Full Course Notes

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Protons flow through atp synthase to generate a conformational change in order to combine adp with pi. Per molecular oxygen reduced, atp is produced, using p/o ratio for nadh and. Fadh2, reduced electron carriers that donate electrons to etc. Electrons flow to oxygen to produce h2o molecule. Protons are pumped across the inner mm to produce atp: we"ll calculate how many atps are made from the breakdown of one glucose molecule, and water molecules as well. 19. 5 19. 7: review questions posted on blackboard, socrative room # 317683. Impermeable imm where all the proteins are present (need intact membrane) From nadh, 10 h+ are pumped across the imm. From fadh2, 6 h+ are pumped across the imm intermembrane space. Protons flow through atp synthase, inducing conformational change to create phosphoanhydride bond to produce atp from. Adp and pi, as well as a water molecule: need approx. The rotary catalytic mechanisms of mitochondrial atp synthase.

Related Questions

1. The P/O ratio formitochondrial oxidative phosphorylation is the number of ATPmolecules that are synthesized for each two electrons that enterthe electron transport pathway. Under normal conditions, the P/Oratio is approximately 2.5. [The derivation of this number will beexplained in class, though it is not necessary for you tounderstand the derivation to answer this question. Just know thatthe P/O ratio is the number of ATPs made for each NADH that isoxidized]. (a) Uncouplers cause the mitochondrial membrane tobecome permeable to protons, and so uncouple ATP synthesis fromelectron transport. What would be the effect of a low concentrationof an uncoupler on the P/O ratio? Note that a low concentration ofuncoupler will only partially dissipate the proton gradient. (b)DCCD is a reagent that inhibits ATP synthase by blocking the protonchannel through the enzyme. What effect would DCCD have on the P/Oratio? Tip: it is important to understand that inhibition of ATPsynthase also inhibits electron transport, because an increase in[H⁺] in the inter-membrane space exerts an inhibitorybackpressure on the proton pumping complexes (ie rememberthat ATP synthesis and electron transport are coupledprocesses). (c) If mitochondria are inhibited with DCCD, and thenan uncoupler is added, what will be the effect of the uncoupler onATP synthesis, oxygen consumption and the P/O ratio? Explain yourreasoning behind all answers.

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BCH210H1 Lecture Notes - Lecture 30: Affinity Chromatography, Electrochemical Gradient, Intermembrane Space

BCH210H1 Full Course Notes

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