EESB16H3 Lecture Notes - Lecture 9: Flavr Savr, Polyethylene Glycol, Micromanipulator
8 Apr 2015
School
Department
Course
Professor
Document Summary
A cell is totipotent if it has the ability to direct its development into a full adult multicellular organism. Plants cells are totipotent: cells taken from leaf, stem, root, flower or other parts of the plant can be made to dedifferentiate in the lab to callus tissue, and to rediffrenciate again into a complete plant. Dna constructs are injected through plant cell wall into nucleus. Protoplasts are cells that have has their cell wall removed by enzymatic digestion, leaving only the weak cell membrane. Dna can be introduced by electroporation or use of polyethylene glycol. Dna coasted tungsten or gold beads, or dessicated bacteria are shot through cell wall into plant using a particle gun. Dna must integrate into genome of plant this happens but we are unsure of why. First developed and patented by usda and delta and pineland. Marketed as a method of preventing gene flow between crops and wild varieties.
