BIOC39H3 Lecture Notes - Lecture 5: Immunophenotyping, Immunology, Monocyte

• School: UTSC
• Department: Biological Sciences
• Course: BIOC39H3
• Professor: Bebhinn Treanor

Document Summary

Depending on your protein of interest and labelling it with a flurochrome. Stai(cid:374)s that sho(cid:449) if it"s a s(cid:373)aller (cid:272)ells (cid:448)s a (cid:271)igger (cid:272)ell. Cannot distinguish a b cell from a t cell. I(cid:374) the (cid:272)o(cid:374)te(cid:454)t of leuke(cid:373)ia, (cid:449)e (cid:449)o(cid:374)t (cid:271)e a(cid:271)le to tell if it"s a (cid:271) (cid:272)ell or t (cid:272)ell leuke(cid:373)ia (cid:271)it with immunophenotyping we can. Label our protein of interest with an antibody. Shine light on something, its going to reflect and scatter some of that lght (property of every thing) Fsc = forward scatter (the larger the cell the more it will scatter the cell forwardly) Ssc = more intracellular complex, ex: more granules the cell has , the more the cells will scatter in the side complexes. This allows us to distinguish the different properties in the blood (can distinguish monocytes from lymphocytes etc) All cells emit some level of fluorescence intensity. What this (cid:374)egati(cid:448)e populatio(cid:374) is that does(cid:374)"t ha(cid:448)e our protei(cid:374) of i(cid:374)terest.