BIOC14H3 Lecture 6: BIOC14 Lec 6 notes transcribed.docx
12 Mar 2015
School
Department
Course
Professor
Document Summary
Transgenic approaches: modelling (abnormal) human bhv"s by manipulating candidate genes, try to model symptoms. Genes, neurons (n"s) & behaviours (bhv: identify ns expressing candidate genes, measure their activity, determine their connection pattern, modulate their activity. Genes -> neurons (neuron population expressing a candidate gene) -> bhv. > rna in situ hybridization: experimental method for localizing & detecting specific mrna sequences in preserved tissues, a labeled, complementary rna (riboprobe) hybridizes to target gene sequences, generation of riboprobes: Allen brain atlas - of gene expression patterns in mouse brain: automated high-throughput in situ hybridization system for all mouse genes. Now we can study neuron populations expressing a candidate gene. To study their function, need to: measure electrophysiological activity, determine the connection pattern among diff brain regions, modulate the neuron"s activity (through transgenic approaches) Prerequisite to study neuron properties is the ability to target specific n populations without affecting others. Obtaining genetic access to specific neuron population: methods, transgenic approaches using promoters/enhancers.
