BIOC14H3 Lecture : BIOC14 : Lec 10 : Article 2 - Genetic Dissection of Neural Circuits
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Bioc14 : lec 10 : article 2 discussion (only sections 1 &2) We are looking at the relationship btn genes and behaviors in terms of intervening things (ex: neuron itself as the unit of activity in the brain) Transgenic and knock-out mice for generating mice which lack certain genes or certain genes added: Based on these models we can assess the effect of gene. Therefore, you want some control over when this gene is knocked out/expressed. This kind of technology used in other model organism like drosophila (fruit flies), but mouse = classic model. Dna microinjection into single-cell embryos as a way to produce transgenic mice with multiple copies of randomly inserted dna vs. Specifically targeting certain regions of the genome via homologous recombination to generate replacements at very specific loci. Studying individual genes isn t sufficient for studying how the brain works and studying individual cells is also not sufficient b/c cells work in groups/circuits.