BIOC14H3 Lecture Notes - Lecture 5: Stem Cell, Thymidine, P1 Phage
26 May 2018
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Lecture 5
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•Quantitative genetics
•Components of genetic variance
•Calculating heritability from genetic and
phenotypic variance
•Experimental estimation of heritability
•Using laboratory animals
•Mid-parent offspring regression
•Twin studies
[continued…]
Chapter 8
Lecture 5 Objectives find more resources at oneclass.com
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Chapter 9
Lecture 5 Objectives
•Model organisms
•Characteristics
•Databases
•Modifying model organisms
•Mouse as a model organism
•Generating transgenic mice
•Microinjections
•Use of transgenic mice
•Use of reporter genes
•Cell type specific expression
•Using homologous recombination
•Selecting and using transfected ES cells
•Gene knock-in / knock-out
•Conditional knock-out
•Cre-loxP system
•Examples
•Genome editing using CRISPR/Cas9
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Document Summary
Lecture 5 objectives: quantitative genetics, components of genetic variance, calculating heritability from genetic and phenotypic variance, experimental estimation of heritability, using laboratory animals, mid-parent offspring regression, twin studies. 3 phenotypes : observable characteristics of a trait, physical features and behavioural patterns that an animal exhibits, are caused by a combination of genetic background and environmental contributions. But eye colour and some other stuff is solely caused by genetics. Distribution of birth weight we are looking at the frequency of individuals that fall into these different categories. Calculating variance: mean (average) = add up all the heights and divide by the # of samples that we have. Xi is the ith data point n is the number of data points: variance: degree of variability in a group i. e. spread of numbers (cid:198) larger the value, bigger the spread around the mean.
