BIOB12H3 Lecture Notes - Prophase, Statin, Geminin

89 views22 pages
9 Apr 2014
School
Course
Professor

Document Summary

Biob12h3s: cell & molecular biology laboratory. Fluorescence microscopy module (pdf #9) Fluorescence microscopy is a very powerful technique, which is most often associated with immunofluorescence experiments. The dyes absorb light at one wavelength, and emit light at a longer wavelength. In this way, the subcellular location and distribution of specific proteins or other cellular components can be observed. The fluorescence microscope (also called epifluorescent microscope) is diagrammed below. A beam- splitting mirror (2) will then focus this excitation light on to the specimen containing the fluorescent dyes. Alberts et. al. , 3rd edition, pg 8. The fluorescent signal is not permanent. This type of fading is referred to as photobleaching and can be a serious limitation of this type of microscopy. Such photobleaching can be the result of photo- induced oxidation that damages the fluorescent molecules over time (chemical damage or covalent modification). Several measures are available to combat this effect.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents

Related Questions