BIOB11H3 Lecture Notes - Lecture 2: Enterobacteria Phage T4, Centromere, Biotin
Document Summary
Dna renaturation: complementary single strands of dna can re-associate (reanneal) into a stable double stranded dna molecule, renaturation occurs when denatured dna is allowed to cool slowly. Complementary strands will find" each other and base pairing begins: after renaturation, dna becomes a double stranded helical molecule. We then raise the temperature to denature the dna. Therefore the longer time it will take to renature: plots all have a single symmetrical curve because all sequences are present at the same concentration, chromosomes contain genes in a linear array. They usually make up 1-10% of a genome: highly repeated fractions are usually short sequences (100 bp) that repeat themselves over and over without interruption. This is called tandem repeats: highly repeated fractions arose by gene duplication and can anneal very quickly, satellite dna which is generally found at centromeres and telomeres. This dna is usually 5-500 bp in tandem repeats of up to 100 kb that can form very large clusters.
