BIOB11H3 Lecture 16: BIOB11 – Lecture 16
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Smart pages: figs 14-1, 2, 3, 4, 5, 6, 8, 11. Use radioisotopes to label cultured cells can be used to determine cell cycle parameters. Cell fusion experiments suggest that molecules from one stage of the cell cycle influence the cell cycle in another cell type in a heterokaryon. Mpf is a complex of a cyclin and cyclin-dependent kinase (cdk) which promotes the g2 to m transition. Cdks phosphorylate multiple targets to modify their structure/activity. In higher eukaryotes, many cyclins and cdks exist and complexes b/w them regulate passage of cells through the cell cycle. Yeast temperature sensitive mutants are useful for determining the function of different genes in a cell cycle progression. During mitosis both nuclear and cytoplasmic changes occur to compact the chromosomes, segregate them and trigger cytokinesis. 8: what are the respective roles of cak, wee1 and cdc25 in controlling. Suppose you are labeling a population of cells growing asynchronously w/ [3-h]thymidine.
