BIOB10H3 Lecture Notes - Lecture 6: Green Fluorescent Protein, Fluorophore, Rhodamine
Document Summary
Fluorescence microscopy: uses a fluorescence microscope they detect the location of compounds known as fluorophores. Fluorophores or fluorescent dyes: molecules that can absorb energy of a specific wavelength (uv light) release a portion of this energy as longer visible wavelengths of light, called fluorescence. Fluorescent dyes: work only if you shine a uv light on it the wavelength emitted is always higher than the wave length absorb. So, you have your fluorescent dye attached to something that you are interested in. How can these dyes be made to bind different molecules: a fluorophore could be attached to an antibody that is directed against a specific cellular protein. = immunofluorescence: green = dna (fluorescein based dye, red = cytoskeleton protein (rhodamine based dye) Answer: y-shaped molecule is composed of 2 heavy chains and 2 light chains. Heavy and light chains come together to create antigen binding sites. The differences in the antibody allows it to bind different antigens.
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