BIOB10H3 Lecture Notes - Lecture 3: Glycosylation, Dolichol, Oligosaccharide
Document Summary
Keith r. porter lace like network that spread throughout the cytoplasm. Radioactive isotopes release radioactivity when they disintegrate. Include radioactive aa w/ 1 or more radioactive atoms in a growth media the proteins made in the cell will become labeled with these radioactive aa. Ribosomes insert these radio aa into proteins (like normal ones) Synthesize aa w/ radioisotopes cell will make radioactive proteins with radioactive amino acids. Energy emitted from radioactive atoms used to label proteins can be used to activate a radiation sensitive emulsion. Silver grains are visible in the em grains show the location of the proteins in the cell. Dark patches are where the proteins are. Albert claude and palade labelled proteins w/ radioactive isotopes and detected them by autoradiography to determine fxn of endomembrane system: experimental setup. Pancreatic acinar cells (digestive enzymes) added radio. Aa to medium radio aa incorporated into proteins (digestive enzymes) fixed cells used autoradiography and em.
