BIOB10H3 Lecture Notes - Lecture 3: Unfolded Protein Response, Proteasome, Cystic Fibrosis
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Dna is a helix because the phosphate backbone is hydrophilic and nitrogenous bases are hydrophobic. Nitrogenous bases want to be buried on the inside and so there is a close stacking of the bases (causes double strand) The phosphate backbones of each strand have negative charge which repel e/o and cause the natural helix structure (don"t want to be too close to each other competition) Single stranded dna can also adopt a helical structure. Early em revealed that there were organelles inside cells. Em pictures don"t tell us what organelles within endomembrane system do. * after 3 min of this saw that there was radioactivity in the er of the cell proteins were in the cell: cells were pulse-labeled (or short period of time)--> to see where the initial site of. 2. protein synthesis is pulse-labeled with radioisotopes then washed medium with any radioactivity and. * proteins move from er golgi outside of cell.
