BIOA01H3 Lecture Notes - Lecture 2: Ethidium Bromide, Dna Replication, Okazaki Fragments
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After 2 rounds of replication in a labelled medium, one daughter molecule is half labelled, while the other is fully labelled (first rep mixed n^15 and n^14, next replication mixed both n^14 isotope stained dna) Conclusion: dna is semi-conservative as it showed a hybrid daughter molecule. Direction of the chain grows 5"-3" and is carried out by. Dna replication is different for each strand, it is continuous and discontinuous. Unwinding of the dna creates a replication fork. B/c rep. al(cid:449)ays happen 5"-3", top strand elongates in segments, bottom elongates in on continuous motion. Important because dna polymerase does not create nucleotides can only elongate existing piece of. Dna/rna in lagging strand, it is synthesized in discontinuous dna the 2 fragments that are synthesized are called okazaki leading strand has polarity such that after 1 primer, Dnap synthesizes in 1 motion synthesis, and fragments. >> eventually fill and then link the 2 fragments.
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