FSC239Y5 Lecture Notes - Lecture 9: Electrospray Ionization, Microfluidics, Mass Spectrometry
Document Summary
A 10,000 year old body found and were still able to find toxins in the body. Goals: to identify important molecules and to separate important molecules. Mixture of compounds that are able to move in the liquid to separate compounds. Volatile: take compound and turn into gas. Non-volatile: carrier gas tank, flow regulators and into oven to separate the compounds. Microfluidics- caliper technologies (agilent: once the scale is changed, the tension of water will keep it separated, capillary electrophoresis. Compounds will go from cathode to anode. Takes the molecules of two samples, hit it with energy and allow the samples to separate. Very sensitive: can find traces of container in a substance, ex: find traces of cup in coffee. Matching energy of a photon to a process. Any colour is visible because material absorbs the visible colours compliment. Certain substances can absorb x-rays while others don"t. The resonance conditions: the x-ray can rip out the electrons from the orbital.