PHA 4107 Lecture Notes - Lecture 4: Vascular Endothelial Growth Factor, Receptor Tyrosine Kinase, Sh3 Domain
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These receptors undergo autophosphorylation (catalyze their own phosphorylation) and can transphorylate other proteins in response to a ligand. Classes of receptor tyrosine kinases (rtks: epidermal growth factor (egf-r) receptor family includes, egfr1 (erbb1, egfr2 (erbb2, her2/neu, egfr3,4 (erbb3,4 or her3,4, insulin receptor, platelet-derived growth factor (pdgf, fibroblast growth factor (fgf, vascular endothelial growth factor (vegf) The interaction of the external domain of a receptor tyrosine kinase with the ligand, often a growth factor, up regulates the enzymatic activity of the intracellular catalytic domain, which causes tyrosine phosphorylation of cytoplasmic signaling molecules. Grb2 is an adaptor protein containing both sh2 and sh3 domains. How do you achieve specificity of protein-protein interactions: different sh2 domains have different affinities for a given phosphotyrosine (surrounding sequences can confer specificity) The sequences c-terminal to the consensus phosphotyrosine binding domain determine if a protein will bind to an rtk and (if it is a substrate) be phosphorylated.