HSS 1100 Lecture Notes - Lecture 8: Cell Culture, Sperm Donation, Croup

• School: University of Ottawa
• Department: Health Sciences
• Course: HSS 1100
• Professor: Franco Pagotto

Document Summary

All level 4 pathogens are all viruses. What kind of microscopy do we need when we look at a virus: electron microscopy. How to viruses replicate: know the 5 steps (labelled on the pictures) **exam question** Need to replicate the nucleic and protein synthesis. #4 is asse(cid:373)(cid:271)ly a(cid:374)d (cid:373)atu(cid:396)atio(cid:374) (do(cid:374)"t fo(cid:396)get (cid:373)atu(cid:396)atio(cid:374) othe(cid:396)(cid:449)ise you"ll lose marks) How do we detect viral infections: 1. Dete(cid:272)t the i(cid:373)(cid:373)u(cid:374)e (cid:396)espo(cid:374)se a(cid:374)ti(cid:271)odies agai(cid:374)st the (cid:448)i(cid:396)us: send blood work and do an elisa test. Viral diagnosis (can be done in 3 ways: a. detection in clinical specimens, 1. Visualization by electron microscopy: you prepare it, put it under the electron microscope and see (cid:449)hat"s good, 2. Cell culture (cytopathic effects, hemagglutination, immunofluorescence: b. Detection of patie(cid:374)ts" immune response: antibody detection, presence of absence (elisa) Immunity test: rise in antibody titre or high antibody titre, diagnostic test, rmr that a direct elisa detects ag, rmr that an indirect elisa measure the host response (ab)