CHM 1321 Lecture Notes - Lecture 1: Thin-Layer Chromatography, Ethyl Acetate, Silica Gel
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22 Mar 2015
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One of the types is called thin layer. Tlc involves a very thin layer of sorbent layer on top of a material such as glass, plastic or aluminium for support (snyder, kirkland & dolan, There are many different kinds of sorbent layer that can be used, but a few common ones include silica gels, cellulose, and aluminium oxide. Samples are then applied in spots to the sorbent layer. Next, an eluent is allowed to flow through the sorbent, starting at a point just inferior to the spots where the samples were applied. This is achieved by placing the eluent in a jar or glass box where the eluent is about 5mm high and after the sorbent plate is placed within it, a lid is placed on top to prevent evaporation. As the eluent front moves up the sorbent layer, the samples do as well.
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