BCH 4101 Lecture Notes - Lecture 10: Zinc Finger, Genome Editing, Dna Annotation
12 Dec 2019
School
Department
Course
Professor
Document Summary
Affordable whole-genome sequencing + detailed databases of genetic variation + large genome annotation. Can get inside the cell - that way on works for things that work extracellular. Try and make the correct protein that is missing etc. Rnai - prevent translation of mrna product. How to make sure you get enough of the suppression. Off-target effects - only make sure you only target what you want to. Safety - dna won"t integrate within another protein and cause more. Universal way of fixing the dna singe majority are single mutations damage, etc. Idea is going in finding the mutation and just fixing that. Based on the idea that we introduce a ds break. Or jam two dna ends together and lose information in the middle. How do genome editing nucleases recognize and cut specific sequences in the genome: zinc finger nucleases. Introduce dna break in a precise location in the genome.
