BIOL 4050U Lecture Notes - Lecture 1: Plasmid, Transfection, Elisa
Electroporation Improves Efficacy of DNA vaccines in Large animals Notes
Two different electrodes:
Single-needle electrode
Six-needle electrode
Plasmid-based vaccines can be considered as a novel approach to immunology
DNA vaccines are able to induce immunity to a wide variety of infectious agents, including
viruses, bacteria and parasites
DNA vaccines do not require a cold chain to maintain the efficacy of the vaccine
DNA vaccinations are safer than using live attenuated vaccines
To investigate the efficacy of in vivo electroporation, the researchers used plasmids encoding
two completely different antigens
Surface antigen (hepatitis B, HBsAg)
Membrane protein (bovine herpesvirus glycoprotein gene gD)
Engerix-B sub-unit vaccine: helps prevent hepatitis B by exposing the animal model to a small
amount of the virus
Site of vaccination was quadriceps of both hind legs
Electroporation facilitates the introduction of foreign DNA into target organism by changing
permeability of the cell membrane
Electroporation enhanced gene expression
Level of antigens expressed correlates with the magnitude of the elicited immune
response
Protein subunit vaccine: only includes the antigens that stimulate an immune response
With no viral particle
Using an adjuvant increases the body’s immune response to the vaccine
Humoral immune response
ELISA: enzyme linked immunosorbent assay
Seroconversion: Period of time during which antibodies develop and become detectable
Deoxyribonucleic acid Vaccine: provide genes encoding the antigens instead of consisting of
antigens
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Document Summary
Electroporation improves efficacy of dna vaccines in large animals notes. Plasmid-based vaccines can be considered as a novel approach to immunology. Dna vaccines are able to induce immunity to a wide variety of infectious agents, including viruses, bacteria and parasites. Dna vaccines do not require a cold chain to maintain the efficacy of the vaccine. Dna vaccinations are safer than using live attenuated vaccines. To investigate the efficacy of in vivo electroporation, the researchers used plasmids encoding two completely different antigens. Membrane protein (bovine herpesvirus glycoprotein gene gd) Engerix-b sub-unit vaccine: helps prevent hepatitis b by exposing the animal model to a small amount of the virus. Site of vaccination was quadriceps of both hind legs. Electroporation facilitates the introduction of foreign dna into target organism by changing permeability of the cell membrane. Level of antigens expressed correlates with the magnitude of the elicited immune response. Protein subunit vaccine: only includes the antigens that stimulate an immune response.
