BIOL 2080U Lecture Notes - Lecture 1: Fractional Distillation, Affinity Chromatography, Boiling Point

71 views4 pages
29 Jul 2017
School
Department
Professor

Document Summary

Extraction steps: add a weak acid: strong bases will be removed, add a strong acid: weak bases will be removed, add a weak base: strong acids will be removed, add a strong base: weak acids will be removed. Extraction: an aqueous layer and a less dense organic mixture. ___ separates based on solubility. -- must be at least different by 20o c lower boiling point means it will be captured off first and condensed into a cool tube. Fractional distillation: a more precise method used to separate compounds with similar boiling points ( so with a difference of less than 20o c) Purpose: to separate based on the principle that pure substances form crystals more easily than impure substances. The (cid:373)ost (cid:862)(cid:272)o(cid:373)(cid:373)o(cid:374)(cid:863) e(cid:454)a(cid:373)ple of (cid:272)r(cid:455)stallizatio(cid:374) is the for(cid:373)atio(cid:374) of i(cid:272)e(cid:271)ergs as salt is excluded from this structure. Used to: purify a compound in the mixture.

Get access

Grade+
$40 USD/m
Billed monthly
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
10 Verified Answers
Class+
$30 USD/m
Billed monthly
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
7 Verified Answers

Related Documents

Related Questions