BIOL 335 Lecture 6: Gene regulation (cont.)
Document Summary
Summary for techniques used for analyzing transcript abundance: Global gene expression analysis: microarray, rna sequencing. Analysis of expression of individual genes: rna blot, rt-pcr / real-time rt-pcr, in situ-hybridization, promoter-reporter gene fusion. Understand the advantages and disadvantages of the four techniques for analyzing expression of single genes. You suspect that transcript of a newly cloned c. elegans gene is located specifically in the developing nervous system. Which of the following techniques would be the best choice to confirm your hypothesis: rna blot, rt-pcr, in situ hybridization, microarray hybridization, promoter-reporter gene fusion transgenic c. elegans. How do we know that transcriptional regulation is a commonly used mechanism for differential gene expression during development: differential distribution of transcripts within a developing organism, transcription (not rna degradation) is the principle mechanism for controlling. The promoter and enhancer regions of genes with specific patterns of transcript localization can be used to express reporter genes in the same pattern.
