BIOL 335 Lecture Notes - Lecture 3: Epistasis, Messenger Rna, Candidate Gene
Document Summary
Sites in the genome that don"t encode for any function. Areas in the genome which might differ by a single nucleotide, which you can detect if you set up your pcr primers right. A cross was made between ag (in col) and wild type ler. In the f2 population, eight plants with agamous phenotype were selected. Pcr was performed on dna extracted from these plants using dna markers for four loci. a, b, c, and d are col alleles. A, b, c and d are let alleles. The results are shown below: a/a a/a a/a a/a a/a a/a a/a a/a b/b b/b b/b b/b b/b b/b b/b b/b. C/c c/c c/c c/c c/c c/c c/c c/c d/d d/d d/d d/d d/d d/d d/d d/d. We know that these all should have the recessive agamous genotype. If this particular molecular marker is linked to the ag then that particular alleles should be inherited all the time.
