BIOL 201 Lecture 21: Quantitative Enzymology
Document Summary
Equilibrium : when forward and reverse reaction rates are equal. Steady state : when [product] and [reactants] remains constant. Because steady-state can be maintained at a point away from equilibrium , the reaction can then be used to do work for the cell. Note: g" is dependent on the [reactants] and [products] at steady state and at equilibrium. Reflects where [products] and [reactants] sit at equilibrium only. Reaction does not have to be at equilibrium, and. Concentrations may not be 1m (will be noted in question) When reaction is at equilibrium, q = k eq ". Can be thought of as the reaction snapshot (instantaneous) Assumes that reaction is being carried out under standard biochemical conditions and reaction is at equilibrium. I. e. reaction must start with 1m reactants and 1m products at 25 c at 1 atm, ph = 7, and h 2 o = 55. 5m. Does not necessarily assume anything about reaction conditions, or the state of the reaction.
