BIOL 200 Lecture Notes - Lecture 29: Tubulin, Microtubule, Cytoskeleton

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17 Nov 2017
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They are hollow tubes of -tubulin heterodimer subunits. Two cases of microtubules assembly: in vitro monomers in a tube. Initiating cytoskeletal polymerization (nucleation) to build microtubule or actin polymers is a slow process in vitro: in vivo inside the cell. Microtubule organizing centers (mtocs) in cells provide the right conditions for rapid nucleation of microtubules. Tubulin dimers have gtp bound -tubulin, but are converted to gdp in microtubule (mt) polymers: tubulin dimers have two forms: Active tubulin dimers gtp bound -tubulin. Inactive dimers are reactivated in the cytosol by replacing gdp with gtp. Structural polarity: -tubulin exposed on (-) end, -tubulin exposed on (+) end. The kinetics of spontaneous microtubule assembly in vitro. Gives the mt a preformed template to start with. Allows cells to preferentially make mts when and where they need them. The rate of polymerization is dependent on: location. Adding new subunits causes a conformational change in -tubulin that increases.

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