BIOC 302 Lecture Notes - Lecture 10: Dna Ligase, Restriction Enzyme, Polymerase Chain Reaction
Document Summary
With every cycle amount of dna grows exponenially. Direcion of synthesis is always 5" 3". Primers: need to be complementary to your dna. The primers" 3" end have to point towards each other. Limitaions: length 2-5 kb works best, ampliied dna is not 100% accurate. To perform pcr, an 18-30bp sequence on either side of the sequence to be ampliied must be known because. Dna pol requires a primer to synthesize a new strand of dna. Whatever is made from the original one will result in the same length ater that. Only the original one can be longer anything ater that will be the same length. Q1: the gel shows results from diferent pcr experiments (one experiment per). Comment on the product proiles of lanes 1,2,3, and 5. 2 = smaller than the irst one = you get more product if you have smaller. 5 = sample dna has more than one site the primers can bind.
