CHEM357 Lecture Notes - Lecture 1: Spectral Theorem, Sedimentation Coefficient, Broad Peak
14 Dec 2016
School
Department
Course
Professor
Document Summary
Sedimentation signals acquired at a rotor speed of 48, 000 rpm at 26 c for stable proteins, with areas under the peak corresponding to concentration. (a) c(s) distribution determined separately for a mixture of aldolase and igg displaying a single broad sedimentation boundary (solid). Absorbance at 250nm (dashed). (b) global multisignal analysis of aldose, Igg mixture displaying two well separated peaks corresponding to each protein component. Dotted lines represent signals from separate experiment igg and aldose alone for comparison. (c) solid displays component sedimentation coefficient distributions from mixture of aldose, igg and bsa. Distributions obtains from individual proteins displayed by dotted lines for comparison. Model for multicomponent mixtures takes advantage of the different extinction coefficients of sedimenting protein components to determine the sedimentation coefficient distribution for each component in solution. Overall, it provides a method to determine the composition of a protein complex.
