BIOL308 Lecture Notes - Lecture 15: Electrophoretic Mobility Shift Assay, Deoxyribonuclease I, Electrophoresis
22 Jun 2017
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1: labeled probe without dna travels the farthest. 2: protein with labels probe makes 2 bands 1 is slower when bound to protein, another is faster when unbound. Use mutants of that cis acting element so the protein won"t bind, therefore producing a different footprint than the wild type and identifying the tf binding site. Tf and promoter: domain: large chunk of protein with a common function (3* structure) Dna binding: domains structural motifs interact with the dna. Effector binding: effectors can affect the conformation of a regulatory protein. Oligomerization: makes a di/tri/tetramer: motifs: inside domains, aa sequences making structures that interact with dna that help the domain function. Helix turn helix: 20aas, part of tf; helix 1 (stabilizes h2) and 2 (recognizes and binds to dna) is frequent in repressors. Lambda phage: helix 3 (stabilizing) and 2 (recognition) in repressor to interact with the dna.
