BIOL308 Lecture 7: Lecture 7- DNA rep (cont)
11 Jun 2017
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Document Summary
Can remove ribonucleotides (including those directly linked to the dna) Fills in the gap with dntps after rna removal (normal 5"-3" polymerization activity. Free 3" oh necessary for polymerization removes faulty nucleotides fills the gap based on template. Will cut away place where error is and replace it. Has ability to back up and remove nucleotide. Rnase h (may only be required in dna replication in some prokaryotic organisms) Rnase h removes most of rna made by rna primase (except the last nucleotide, which is removed by a 5" exonuclease) auxiliary role with longer primers. Rather than dna pol1 eliminating error, rnaseh will remove most other than 1 nucleotide and pol 1 cam come in and remove ribonucleotide and replace with dna. Enzyme that links fragments on the lagging strand. Forms a phosphodiester bond between the 3"-oh terminus and the 5"-po4 terminus of adjacent nucleotides (forms within the strands)
