BIOL303 Lecture Notes - Lecture 20: Transcription Factor Ii B, Tata-Binding Protein, Transcription Factor Ii D
9 Jun 2015
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What is the difference(s) between enhancer and upstream control element: explain the use of reporter genes for estimation of promoter strength, explain briefly 5 deletion series. What kind of information do they reveal: explain the modular nature of rnap ii promoters, draw a diagram of rnap ii promoter (show all types of elements that we have mentioned in class). 10. explain the tissue (cell type) specificity of eukaryotic cis elements. 12. if you know the binding site for certain transcription factor (tf), outline experiments you would use to purify this tf and to assay its activity. 13. distinguish between the function of promoters and enhancers in transcriptional regulation. 14. distinguish between the function of general transcription factors and transcription activators in transcriptional regulation. 15. list and briefly explain four major domains in eukaryotic transcription factors. 16. list most frequent structural motifs in eukaryotic dna binding domains activation. 17. list three classes of transcription factors. domains in eukaryotic transcription.
