BIOL240 Lecture Notes - Lecture 1: Methylene Blue, Enterococcus Faecalis, Enterobacter Aerogenes
15 Mar 2015
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Document Summary
Simple staining: unstained microbes are transparent and hard to see, and must be stained so that there is a contrast between the light and the microbe, all cells stain the same colour in simple staining we can distinguish b/w the different morphologies of bacteria, use methylene blue (blue), basic fuchsin (red), crystal violet (purple) Basic bacterial shapes: bacillus (rod), coccus (round), spiral (spirillium, coccus = mostly non motile, singly (micrococcus), pairs (diplo), chains (strepto), groups of four (gaffkya), groups of 8 (sarcina), clusters (staphylococcus, bacillus = mostly motile, singly, pairs, chains. Stalked forms (caulobacter), club shaped (corynebacterium), comma shaped (vibrio: magnification = (size of specimen in drawing in m) (estimated or actual size of image in m, e. coli = 1. 1 1. 5 x 2. 0 6. 0 m, b. subtilis = 0. 2 2. 5 x 1. 2 10 m, s. epidermidis = 0. 5 1. 5 m wide, rhodospirillium rubrum 0. 57 1. 5 m wide.
