BIOL240 Lecture Notes - Lecture 8: Trp Operon, Camp Receptor Protein, Stem-Loop
12 Jul 2017
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Mutations affect the genetics and regulation of cells. Rna polymerase in bacteria has both a core enzyme and sigma factor it helps it recognize the genes its to transcribe. Different sigma factors can be used by rna polymerase bc the sigma factors are protein components that are modular. One sigma factor can be changed for another. Dna to ensure that transcription begins at the correct location, the holoenzyme recognizes a specific region of. In bacteria consists of 10-35 elements which are located upstream of transcription initiation. Rna polymerase (including the sigma factor) binds to the promoter region at minus. 10 and minus 35 positions (i. e. , the consensus sequence) the site of initiation is referred to as the +1 position and in bacteria typically is an a or a. Rna polymerase + sigma factor = holoenzyme. Two mechanisms of termination within bacteria: rho-dependent, rho independent.
