BIOL239 Lecture Notes - Lecture 14: Methionine, Start Codon, Release Factor
Document Summary
Set 14: translation the process in which the genetic code carried by mrna directs the synthesis of proteins from amino acids requires: mrna, rrna with attached amino acid ribosomes. Wobble: some trnas recognize more than one codon for the amino acid they carry. Ribosomes: sites of protein synthesis, complex structures composed of protein and rna ribosomes have a large and small subunit, the size differs from prokaryote and eukaryotes, prokaryotic small subunit carries a 16s rrna while eukaryotic carries 18s. Translation (eukaryotes): initiation phase: 40s ribosome subunit recognizes and binds to the 5" (cid:373)ethylated cap. It then scans along the mrna until it finds the initiation of translation sequence (which in lower eukaryotes is only the aug, but in mammals, it also includes the surrounding sequences) Initiator trna carries met not fmet: rest of translation is similar to prokaryotes. Polyribosome: a complex of several ribosomes translating from the same mrna.