MBIO 4612 Lecture 6: Eukaryotes Lecture 6

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Proteins 90: histones = rich in basic amino acids. Histones + dna = nucleosome/ nucleoprotein (nucleic acid bound to protein) Surrounded by a double membrane makes it more resistant to destruction than the outer membrane. The ground state of most prok genes is on. The ground state of most euk genes is off. Multiple rna pol"s: pol i 5. 8s, 18s, and 28s rrna, 80% of total transcription, pol ii mrna, snrna, mirna, determines what genes are expressed, pol iii trna, 5s rrna, other small rna. Compartmentalization: nucleus = barrier, ts regulatory proteins must be transported into the nucleus to access target genes, processed mrna must be transported out of nucleus for tl. Dna compacted as chromatin: regulates gene expression what keeps it in the off position vs the on position. Monocistronic transcripts: each mrna encodes only 1 protein (vs proks which have polycistronic)

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