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MBIO 2020 Lecture Notes - Lecture 8: Alanine, Ammonia, Start Codon

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grayrabbit360
10 Nov 2016
School
University of Manitoba
Department
Microbiology
Course
MBIO 2020
Professor
Dibrov Pavel

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Document Summary

Complex of 50s ribosome, 30s subunit, mrna and fmet-trna. Protein synthesis by sequential addition of aa. 3 steps: binding of aminoacyl trna in a site. The next mrna codon is in a site, initiation codon is in p site. This codon is recognized by aa-trna which has complementary anticodon (aaa) As a result, two aa trna are aligned in p and a sites; their aminoacyl portions are located in. Reaction of peptide bond formation - catalyzed by peptidyl transferase - not a protein, it"s a. Fmet in p site forms peptide bond with next aa (lets say, phe for example) in a site. Uncharged trna is released from ribosome in two steps: translocated from p site to e site, release into solute from e site. Dipeptidyl group attached to trnaphe is located in a site: translocation. Ribosome moves down one codon toward 3" end of mrna. Next codon to be translated is now in a site.

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MBIO 2020 Lecture Notes - Lecture 7: Protein Structure, Ribonuclease, Primary Transcript
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Related Questions

Given the wobble rules, what is the fewest number of tRNAs necessary to insert the amino acid Leu into a peptide, and what is the anticodon for each tRNA? There are six codons that specify Leu: UUA, UUG, CUU, CUC, CUA, CUG. Keep in mind all sequences in this question are written 5' to 3'

A) two, with anticodons IAA and GAG

B) two, with anticodons UAA and IAG

C)three, with anticodons IAA, GAG, and UAG

D) three, with anticodons UAA, GAG, and UAG

E)three, with anticodons UAA, IAG, and UAG

A researcher isolates a mutant variant of the bacterial translation factor EF-G. The mutation allows proper folding of the protein and binding of GTP, but does not allow GTP hydrolysis. At what stage would translation be blocked by this mutant protein?

A)Binding of fMet-tRNAfMet to P site.

B) Second aminoacyl-tRNA binding to the ribosomal A site.

C) Formation of peptide bond between amino acids.

D) Movement of ribosome along the mRNA to allow binding of a new EF-Tu–tRNA in A site.