NUTR 3210 Lecture Notes - Lecture 3: Lignan, Coprophagia, Spinach

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Calculated from amount of nutrient in diet and amount appearing in feces get no iron from spinach when you consume it, not in a form body can effectively extract. Represents a combination of nutrient release from food matrix, microbial fermentation, and absorption. Want to know if the quality of food stuff we are feeding is good enough to sustain a healthy diet. *bad assumption = only thing that comes out in feces is waste that body cannot use. Allow animal to adapt to diet over 7-21 day period. Isolate animal for quantitative analyses (measure everything consumed and released) Collect and weigh all feces (typically just feces, but urine should be too) Animals confined in costly equipment (cid:523)social animals don"t want to be isolated = stress = hormone release(cid:524) Accuracy in measuring food intake (vomiting, food falling off, etc. = under estimates true digestibility of protein and fat) Metabolic cages = anxious animals (collect and analysis urine and feces)

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