MICR 4280 Lecture Notes - Lecture 11: Ribosome-Binding Site, Mesocosm, Generalized Linear Model
19 Mar 2019
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Taxonomic identification: otus sharing 97% identity go into bin 1. Sequence closest to consensus used for database search. Cannot link function to source suing these techniques. Stable isotope probing: analytical technique that can link identity with function. Largely based on nucleic acid studies, however, we can also look at stable isotope labelled phosphlipd-derived fatty acid purification through ms analysis (but this does not work for all organisms: top down approach. Stable isotopes: 13c 15n 18o: need to pruchase substarte of interest witht hese isotopes. 12c 14n 16o: these stable isotopes are not radioactive, cannot do this with just anything (ex sulfate producers with labelled sulfate) Labeled substrates consumed (oxidized or reduced in an assimilatory reaction) incorporation into cellular polymers given increased density compared to those that contain regular isotopes. Start with phospholipids and then analyze the fatty-acids. Incubation with substrate in the mesocosm or the microcosm.
