MBG 3080 Lecture Notes - Lecture 4: Dna Replication, Uracil, Cytosine
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18 Sep 2019
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1. How can scientists use the CRISPR/Cas9 system used to mutate a gene? (Choose ALL correct answers)
[] | Cas9 and the RNA it binds to can be put into cells by scientists in order to induce breaks at specific DNA sequences |
[] | once the DNA is cut, repair mechanisms of the cell can make changes to the DNA at that specific site |
[] | Cas9 binds with the transcribed mRNA from the gene and interferes with translation so that the protein is never produced |
[] | Cas9 can bind to the promoter of the gene to change the frequency of gene transcription |
2. Which of the following are features of the CRISPR/Cas9 system? (Choose ALL correct answers)
[] | Cas9 uses RNA to find matching sequences of DNA |
[] | Cas9 makes double-stranded breaks in specific sequences of DNA |
[] | Cas9 makes random double-stranded breaks in DNA |
[] | Cas9 makes breaks in RNA that are targeted and specific |
DNA Structure and Function LabReport
- DNA Structure
- Which two scientists are credited with discovering DNA?
- Name the nitrogen bases that are purines.
- Which nitrogen base pairs with thymine?
- List the three components of a nucleotide.
- DNA Replication
- What is the purpose of DNA replication?
- How many times does replication occur in the life of acell?
- In the Lab, Exercise 2, the original strand on the left had thebases shown below. Input the new bases that correctly pair with theoriginal strand.
Original | New |
C | |
A | |
G | |
T |
- RNA Structure
- Describe the structure and function of RNA.
- Refer to Exercise 3 and record the bases of the RNA strandproduced from the replicated DNA strand.
DNA | RNA |
C | |
A | |
G | |
T |
- Record the differences between DNA and RNA in the tablebelow.
DNA | RNA | |
Sugars | ||
Bases | ||
Strands |
- RNA Synthesis
- The process of assembling RNA is called _________.
- How is replication different from transcription?
- Refer to Exercise 4. Write the letters for the base sequence ofmRNA in the spaces below DNA. Note that the order is reversed;start with the 3â end of the DNA strand and the 5â end of the mRNAstrand. Transcription is DNA to mRNA. Note RNA contains Uracilinstead of Thymine; There is no thymine in RNA.
DNA | 3â | C | G | T | C | G | T | C | C | A | A | T | T | 5â |
mRNA | 5â | 3â |
- Protein Synthesis
- What type of RNA provides amino acids to build polypeptidechains?
- If a mRNA strand has the bases 5â CUC 3â, what amino acid willbe translated? Refer to the printable chart in Exercise 5.
- Where in the cell does translation occur?
- Genes
- What could be the problem if there is a change in the basesequence of a gene as it is passed down to the offspring?
- Give an example of a disorder that results from changes in theamino acid sequence.
- What causes sickle cell anemia?
- Gene Cloning
- What is the function of a plasmid?
- Print the document from Lab, Exercise 6. Complete the activityalong with the video demonstration. Sign, date, and take an imageof your final product and include with this lab report.