MBG 2040 Lecture Notes - Lecture 19: Complementary Dna, Central Dogma Of Molecular Biology, Proliferating Cell Nuclear Antigen
8 Sep 2018
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Mbg*2040 foundations in molecular biology and genetics (fall 2017) Instructors: dr. j. uniacke [lectures 16-18] and dr. v. bamm [lectures 19-30] Anti-parallel strands (i. e. always run in opposite directions) Complementary base pairs (a pairs with t; g pairs with c) Right-handed double helix (b form dna): the most common form in cells of living organisms. The prokaryotic oric region: coli origin (oric) is 245 nucleotide pairs long and contains two conserved sequence repeats: 13-bp - 3 repeats - a:t rich - forms replication bubble (replication bubble is essential 1st step of replication) 9-bp - 4 repeats - interspersed with other sequences - binding sites for proteins that help form the replication bubble (mediates protein binding to form replication bubble) The escherichia coli chromosome contains approximately 4 x 106 (4 million) nucleotide pairs and replicates as a single bidirectional replicon in approximately 40 minutes under a wide variety of growth conditions.
