BIOL 2400 Lecture Notes - Lecture 3: Dna Paternity Testing, Genbank, Gang Of Youths
24 Feb 2015
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Separation of dna molecules by agarose gel electrophoresis. Are there homologous sequences in the genomes of other organisms? genomic dna restriction enzyme digestion separate fragments by gel electrophoresis. Two steps: transfer and immobilize the dna fragments from an electrophoretic gel onto a membrane, detect the presence of specific dna fragments through molecular hybridization. Annealing of a dna/rna strand to its complementary dna/rna stand. It requires prior denaturation (separation of the two strands of the dna or dissolve the regional double-stranded structure with the rna molecule, either by heat or chemical treatment). double stranded dna. 5"! hybridize (anneal) radioactive probe to its complimentary strand. Transferring dna from a gel to a dna- binding membrane. A southern blot gives information about the number of fragments containing homologous. Nitrocellulose membrane with bound dna fragments from agarose gel was subsequently incubated with a radioactive dna probe that hybridized to a specific. Dna sequence and then washed and exposed to x-ray film. http://www. bio. davidson. edu/courses/genomics/method/southernblot. html.
