BIOL 1090 Lecture Notes - Lecture 12: Transfer Rna, Start Codon, Methionine
11 Mar 2016
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Recall: diferences in the iniiaion, elongaion, terminaion and processing of transcripts. 10 and -35bp regions upstream of start site. Several genes can be transcribed on one mrna transcript. Terminaion requires a hairpin sequence followed by 6 a"s, polymerase falls of. Tata box and caat bos ~20 and -80 bp upstream of mrna start site. Transcripion factors bind dna, which then recruits the polymerase. Transcripion occurs in the nucleus, translaion occurs in the cytoplasm. Polymerase recognizes terminaion sequence aauaaa, and the mrna is cleaved at downstream gu site. Translaion: messenger rna molecules are an intermediate between dna ad protein. Note: prokaryotes do not have a nucleus, so think about how transcripion and translaion would be diferent between prokaryotes and eukaryotes. In prokaryotes, an rna sequence posiions the ribosome to begin translaion at the beginning of a coding sequence or open reading frame. This begins at the shine-dalgarno sequence to iniiate translaion.
