BIOL 1090 Lecture Notes - Lecture 7: Non-Coding Rna, Nuclear Pore, Light-Independent Reactions
28 Jul 2019
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Intracellular calcium handling: lot energy to control changes in ca2+ conc, ca2+ excluded from cytosol, ca2+ doesn"t bind to water (would cause phosphate ppt, insoluble proteins) Cytosol calcium bound by ca2+ binding proteins. Protein synthesis review: transcription (dna rna): rna polymerase reads dna, produces rna (complementary, antiparallel) Starts at promotor region, continues until terminator sequence. Polymerase in 5" to 3" direction : rna processing: modified in nucleus, mature mrna, noncoding rna. Mrna gets 5" cap, poly a tail, leaves nucleus. Noncoding rnas don"t encode proteins (trna, rrna) transcribed from nucleolus dna: transport: mrna nuclear pore cytoplasm (all before in nucleus, translation: ribosomes translate mrna in cytosol polypeptides. Ribosome scans for aug start codon, starts translation stop codon. Uncharged trna on e-site, ejected: new trna complementary to next codon binds on a. Functions of rough er: synthesis of membrane phospholipids, protein glycosylation: adding carb chains to proteins, quality control of protein folding. Molecular chaperones help in folding: protein synthesis, modification, transport:
