BIOC 3560 Lecture Notes - Lecture 9: Glycolysis, Glycogen, Pyruvic Acid

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 3560
• Professor: Manfred Brauer

Document Summary

Steps in chemical reaction: carbamoyl phosphate (cp) binds to atcase. This creates and asp binding site: asp binds atcase. Large changes in quaternary structure (t r transition: condensation reaction, n-carbamoyl-l-aspartate (ca) released, pi released. (only need 1 of the 6 catalytic subunits to bing both substrates for t r transition. **in the absence of the negatively charged r group, or the positively charged r group (this depends on the amino acid), an ion pair that stabilizes the t state is eliminated. In mutant without salt bridge, r state is favoured. In the presence of a ligand, wt and d236ac are very similar, without the ligan they are different. Conclusions: d236ac mutant eliminates an ion pair that forms between d236c and. K143r: this ion pair normally stabilized the t state, in the absence of this interaction (which connects the c and r subunits), It is generally in a partially active conformation: no allosteric regulation frozen on.