BIOC 2580 Lecture Notes - Lecture 3: Chloroform, Dihydroxyacetone, Biomolecule
21 Oct 2016
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Need to separate fas from glycerol in order to identify. Fas separate into mobile and stationary phases based on properties. Step 2: use absorption chromatography or thin-layer chromatography. Step 4: gas-liquid chromatography or high-performance liquid chromatography. Step 5: separated fas can be definitively identified via mass spectrometry. Built from a limited number of building blocks (#of possible sugar molecules is huge) Monosaccharide"s = simple sugars = single sugar unit = glucose. Polysaccharides = polymer of 20 or more sugar units = glycogen, cellulose. Poorly soluble or insoluble in organic solvents such as ether or hexane. Carbonyl c=o group, which is either an aldehyde or a ketone and. At least 2 carbons bearing hydroxyl (alcohol) -c-oh. The simplest monosaccharides contain 3 carbon atoms (trioses) Each of these groups have aldoses and ketoses = aldotriose and ketotriose. *hexoses (d-glucose, d-fructose) are the most common monosaccharides in nature. *glycerol is not carbohydrate because it doesn"t have a carbonyl group.
