ANSC 4050 Lecture Notes - Lecture 5: Myc, Neomycin, Somatic Cell Nuclear Transfer
18 Sep 2017
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Document Summary
Types of stem cells: embryonic stem cells (escs) grown in the lab from cells found in the early embryo, tissue stem cells found in our bodies all our lives adult stem cells. Identifying methods to control their differentiation and growth is challenging. Immune rejection: human and mouse embryonic stem cells have different properties, risk of teratoma formation, ethical issues. Advantages and disadvantages of es and adult (somatic) stem cells: embyronic stem cells, advantages, high plasticity (pluripotent, teratoma formation use to test pluripotency (marker, easy to grow and expand. Infinite self renewal potential: disadvantages, ethical issues, kill embryo t get stem cells, legal issues, adult stem cells, advantages, less moral issues, less legal controversy, disadvantages, limited plasticity (multipotent, harder to isolate, grow and expand, shorter proliferative potential. Plan to test whether they are indeed stem cells in vitro. Induced pluripotent stem cells (ipscs) state: dedifferentiated from adult somatic cells in vitro, forced expressed of a set of stem cells pecific genes.
