CMMB 549 Lecture Notes - Lecture 23: Single Molecule Real Time Sequencing, Sanger Sequencing, Pan-Genome
28 Dec 2019
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Genome: the entirety of an organism"s hereditary information (can be dna or rna) Core genome: the genes that are present in all sequenced strains of a species. Accessory genome: genetic elements that make species different. Pangenome: genes encoded by the genomes of all strains of a species. Every time you sequence a new genome, you get a few more genes in the pangenome, so the total of genes in pangenome would go up whereas for core genome, it decreases. Breakthrough in sequencing technology: maxam gilbert, a type of chemical sequencing. Each base has distinct chemistry, so you can use different chemicals to cleave the different bonds of each base. So chemicals can breaks bonds at certain locations so you can get identity of base. Maximum length of dna that can be sequenced with this method is 100 base pairs. Sanger won more than 1 nobel prize: sanger sequencing and finding structure of insulin.
