CMMB 461 Lecture Notes - Lecture 13: Reverse Genetics, Genomic Library, Forward Genetics
13 Oct 2018
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Mutate the organism and look for a phenotype. Example: expose flies to mutagenic agent that creates mutations, and observe the phenotype. After mutating the organism, get a cool phenotype. Then you need to find the gene that was mutated. Pinpointing the affected gene is a very tough task disadvantage of forward genetics. Phenotype driven screen: creating mutagenizing cells and looking for specific phenotype. Aim: determine the identity of gene causing the mutant phenotype. You have the genome sequence (so you know what the genes are), mutate specific genes and look at the phenotype. Systematically knock out genes, and observe the phenotype. Gene driven: know the genes, perturb them and observe the results. Identify an interesting phenotype that addresses the biological problem. Mutate genomic dna (chemical mutagens, dna insertions) Identify individuals exhibiting desired phenotype (represent multiple genes involved in the same biological process/molecular pathway) Identify the genes that were mutated (very laborious)
