ZOOL342 Lecture Notes - Lecture 5: Voltage Clamp, Reference Electrode, Electrophysiology
18 Jan 2019
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Voltage clamp: axon within the bath, and an electrode inside the axon and a reference electrode in the bath. And to change the membrane potential there is another electrode inside the axon to add current which is connected to an ampli er. The difference between the set and resting potential is set by the ampli er. Once the cell is sitting at -10, voltage gated channels open, these biological actions occur would further cause other actions. The cell, is always keeping an equal charge, and keep the voltage constant and measure any current across the membrane. K have to leave the cell to become -90 from -60mv. K will go into the cell to raise the potential up to -90mv: by convention, positive charge moving in, is negative current and so negative charge moving out. Voltage clamp experiments: change the membrane potential to more positive, depolarized the axon and maintain it.
