MMI436 Lecture Notes - Lecture 4: Influenza A Virus Subtype H3N2, Neutropenia, Neutrophil
4 Apr 2016
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Document Summary
Neutrophil trails guide influenza specific cd8+ t cells in the airways. Iv tpm- infrared penetrates tissue- two infrared photons excite fluorophore- emission goes through detector- you get a much thicker section analysis- can be use like immunofluorescence but you can do it with thicker tissues. Local and systemic effects of neutrophil depletion in the monoclonal treated mice: no significant difference in the number of neutrophils in the spleen but in the local tissues: lung and trachea: less t cells. Figure 3: what is being released? microarray- detected all the chemokines released: found 9 released from neutrophils, 6 interacted with t cells. + control get 3 epitopes> are they going to add up more than the total t cells. > total t cells: 6,000 total specific t cells: 2,400: very close to total > weird!! almost half of the total t cells are specific*
