MICRB316 Lecture Notes - Lecture 18: Short-Chain Fatty Acid, Pseudomonas Putida, Gene Knockout
20 Mar 2016
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Document Summary
One of the mechanisms that are part of mar response: elux, porins and cytoprotection. Gram negative bacteria have long been known to be more resistant to antibiotics than g+ bacteria (elux is also present in g+). Although you have permeable cell wall without acive transport you will reach equilibrium. However, elux pumps working in conjuncion with a relaively impermeable membrane will greatly reduce anibioics in the cell. The combinaion of restricted entry + acive drug excreion funcions to limit drug accumulaion in the cell protecion. 5 classes of elux: mfs- major facilitator superfam, abc- atp binding cassete family, rnd- resistance/nodulaion/division h+-dep (a lot to do with resistance not a lot to do with nodulaion/division. On the inner membrane of g-, h+ drive exports of pump: smr- small mulidrug resistance, mate- mulidrug and toxic compound extrusion. Originally found in g- but also in g+; the rnd family is now found in all major kingdoms.
