MICRB315 Lecture Notes - Lecture 3: Affinity Chromatography, Cell Disruption, Flocculation
18 Oct 2015
School
Department
Course
Professor
Document Summary
Integrating dsp with fermentation; in situ and ex situ. Mechanical grinding c. sonication d. chemical or enzymatic lysis. Product recovery: just a few examples of many a. 1 and 2 metabolites: solvent extraction; dialysis; chromatography b. proteins: precipitation; affinity chromatography. Nished the upstream ones already l i y g o o b o r c. At the end, will need to extract product from aqueous medium. French press - put cells in high pressure. Got biomass, product in fermenter what to do now. Gonna do: in site - doing product recovery in the. Continuous perfusion, vinegar production - let it drain ex site - turn of all the stuff and do work outside of fermenter then decide how to separate cell: Ef uent - waste product - need a plan to how to handle that, recycle. Try to get ef ciently, reproducible, recovered product. Low volume, high value - doesnt have to be as pure.
